[HTML][HTML] Contacts between extracellular loop two and transmembrane helix six determine basal activity of the thyroid-stimulating hormone receptor
G Kleinau, M Claus, H Jaeschke, S Mueller… - Journal of biological …, 2007 - ASBMB
A number of alanine mutations in extracellular loop two (ECL2) of the thyroid-stimulating
hormone receptor (TSHR) were found to increase or decrease basal activity when compared
with the wild type receptor. K565A was identified as a mutant with decreased basal activity,
and strongly impaired hormone induced signaling activity. To gain insights into how ECL2
mutants affect basal activity, we focused on constitutively activating pathogenic mutant I568V
in ECL2, which exhibits elevated basal activity. Because our molecular model suggests that …
hormone receptor (TSHR) were found to increase or decrease basal activity when compared
with the wild type receptor. K565A was identified as a mutant with decreased basal activity,
and strongly impaired hormone induced signaling activity. To gain insights into how ECL2
mutants affect basal activity, we focused on constitutively activating pathogenic mutant I568V
in ECL2, which exhibits elevated basal activity. Because our molecular model suggests that …
